Results

Results from our commercial clients and academic collaborators clearly validate LCGC's data mining algorithms. OPS-HTS is a powerful formatting technology when applied knowledgably and appropriately for a wide variety of assay formats and readouts. Many working with us have obtained potent and efficacious leads from the LCGC library and OPS. Confirmation retests following pooled HTS consistently yield results as good as, or better than, n = 1 screening (as supported by detailed data from two peer-reviewed published studies). In biochemical assays, the average hit confirmation rate we observed was 74% and in cellular assays this was 89%.

Assay Z-Factor Cutoff Actives Best Potency Retest Rate Endpoint Site Disease Format # Screened
DNA Binding 0.72 20% 63 ‹1 µM 77% ELISA Institute HPV Biochem. 96K
Proprietary Kinase 0.55 50-70% 48 ‹10 µM 66% FP Biotech Diabetes Biochem. 104K
Cathepsin X 0.74 50% 64 5 nM 88% FP Pharma Inflam. Biochem. 64K
Proprietary Protease 0.45 85% 140 500 nM 56% FL (prompt) Biotech Cancer Biochem. 64K
Gprotein AlphaScreen 0.94 85% 18 ‹2 µM 83% SPA University Pain/Epilepsy Biochem. 8K
Chemokine Receptor 0.72 50% 7 5 nM 100% FLIPR Pharma Inflam. Cell 64K
Nfr2-LUC 0.75 30% 16 440 nM 96% LUC University Nephropathy Cell 16K
Infectious Vector R ›0.7 90% 143 ‹1 µM 88% viability University World Health Cell 64K
Chaperone Protein 0.70 150% 64 ‹1 µM 72% LUC Institute Cancer Cell 96K